ABSTRACT The biology and ecology of southwestern Atlantic loliginid squids have been intensively researched in the last few decades, mostly off the Brazilian southern coast. However, information gathered by scientific research cruisers, either past or recent, is limited. Three species of loliginid squids - the warm-tempered Doryteuthis sanpaulensis (Brakoniecki, 1984), plus the tropical D. pleii (Blainville, 1823) and Lolliguncula brevis (Blainville, 1823) - were sampled along 16 degrees of latitude on the southwestern Atlantic (22-38°S). The samples were obtained mostly from oceanographic surveys, but also included squids caught by commercial fisheries, and a few specimens from museum collections. Squid response to abiotic variables, morphological variation and circadian behaviour were surveyed in detail. Doryteuthis sanpaulensis can be divided into at least six distinguishable geographical groups, which do not form a cline. In contrast, D. pleii can be divided into only two morphological groups that are very similar. Doryteuthis spp. were heterogeneously distributed on the shelf, whereas L. brevis was confined nearshore. Our data extended the southernmost distribution range of D. pleii by at least nine degrees of latitude, owing to specimens obtained at ~38°S (Mar del Plata, Argentina). Small, immature D. sanpaulensis were sampled inside the Patos Lagoon estuary (~32°S). The morphologically similar Doryteuthis spp. apparently avoid direct competition by concentrating at different depths, displaying different thermal preferences, and inverse circadian levels of activity. The information reported herein may be regarded as a “snapshot” of the ecology of sympatric squids in a marine environment that has not been deeply affected by climate change.

Storage reservoirs are a key element in wastewater treatment trains for agricultural reuse; however, there is a need for further research on design criteria and operation rules for such reactors. The percentage of fresh effluents (PFE) is an important parameter for the design of perfectly-mixed reactors. PFE correlates better than mean residence time with the performance of the reactor. It allows for estimation of the removal of pollutants in non steady-state systems, such as seasonal wastewater storage reservoirs, and for forecasting the quality of the effluents released for irrigation. However, calculation of PFE is a difficult process requiring complex computer algorithms. A simplified analytical approach is developed to calculate the PFE for n days. The formulation is discussed, describing the relationships between the hydraulic variables, and then applied to a non-steady-state continuous-flow wastewater reservoir in Eastern Sicily (Italy).